Handbook of Molecular & Cellular Methods in Biology

Provides simplified, reliable, and practical step-by-step protocolsPresents the most up-to-date molecular and cellular methods and techniques, including additional methods for analysis of DNA-protein interactions by DNA footprinting and gel retardation assay, mRNA differential display, and microarray technologyClearly explains the principles behind each of the methods presentedIncludes helpful trouble-shooting guides Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era.Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study.Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes:Ë New protocols in all chapters, including alternative protocolsË In vitro transcription methodsË Analysis of DNA sequencesË New bioseparation techniquesNew chapters covering:Ë mRNA differential displayË Inhibition of gene expressionË In situ hybridization (Localization of gene expression)Ë Combinatorial techniquesË Computational data mining methods applied to combinatorial chemistry librariesWith this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.