The book is aimed at molecular bioscientists who want to apply the ever more powerful single molecule imaging techniques for the study of biological systems. Building on successful examples, the new possibilities inherent in single molecule functional analysis are explained. Examples cover the most relevant model systems for this technology, including ion channels, molecular motors, membrane receptors, DNA-binding proteins. Methods encompass spectroscopic as well as microscopic and electrical techniques, for a full spectrum of current high-resolution bioanalytics.