Rapid Detection and Characterization of Foodborne Pathogens

The book puts a particular focus on genes associated with pathogenicity used in PCR, including real-time PCR for specific detection of pathogenic bacteria and the inherent limitations of such methodology with certain pathogens. It also emphasizes methods for extracting microorganisms from complex food matrices and DNA purification techniques. The coverage begins with a highly comprehensive review of real time PCR, complete with theoretical and operational concepts. Each chapter deals with a specific organism and the techniques applied to that organism. The text includes references on the use of PCR primers and DNA probes, the DNA sequence of each being listed at the end of each chapter to create a complete compendium. * Contains a comprehensive description of phenotypic characteristics used for molecular techniques
* Includes an extensive tabulation of primers and probes used with respect to each organism
* Presents the contemporary studies involving the molecular detection, quantification, and subspecies differentiation of each organism
* Provides objective assessments of the various molecular techniques, their advantages, and limitations
* Supplies coverage on microorganism of global interest
* Discusses the level of detection sensitivity of each method and technique