Since the publication of the first edition, the techniques and experimental approaches employed in the study of adipose tissue have undergone radical expansion and revision. In Adipose Tissue Protocols: Second Edition, several updated chapters are combined with many new contributions from internationally recognized experts covering both conventional and state-of-the-art methodologies as well as whole animal, cellular and molecular approaches which have emerged in or been extended to adipose tissue research over the past few years. As a volume in the highly successful Methods in Molecular Biology series, each chapter includes an introduction to its subject matter, a list of necessary materials, step-by-step, readily reproducible laboratory protocols, and a Notes section, which provides invaluable tips and tricks to safeguard against potential mishaps and pitfalls.